Description

The genus Cladosporium is the largest member of dematiaceous hyphomycetes. C. sphaerospermum is a saprophyte that can be found in diverse environments such as indoor and outdoor air, soil, hypersaline water, decaying vegetation, paint, silicone and textiles (1). It has also be isolated from the soil of an aged gas manufacturing site rich in polycyclic aromatic compounds (PAH) (2) and radioactive contaminated wall and soil at the damaged nuclear power plant in Chernobyl (3). The ability of this fungus to survive in such extreme environment possess its potential to become a bioremediation agent as it able to degrade PAH and radioactive compounds.

Few cases of C. sphaerospermum causing phaeohyphomycosis has been reported. This fungus was found to cause subcutaneous phaeohyphomycosis (4), intrabronchial lesion (5), human corneal ulcer and onychomycoses (6). Furthermore, C. sphaerospermum is also known as an allergen (7). The strain in this project, UM 843 was isolated from the peripheral blood sample of a patient in our mycology diagnostic laboratory.

 

Morphological

Flat, radially furrowed with crater-like structure colony was observed on Saboraud Dextrose Agar (SDA) and the colony grew into the agar. The colony was olivaceous green in color and black-green on the reverse side. Microscopically, the conidia of C. sphaerospermum were brown to dark brown(a); globose to subglobose in shape with prominent scar at the end of the conidium(b). Presence of ramoconidia(c), i.e conidia with more than one hilum is one of the characteristics of Cladosporium spp (8). The hyphae of C. sphaerospermum were septated with thick and darkened septa. Under the SEM, the conidia were coronate with protuberant central convex dome surrounded by a raised periclinal rim(d). The ornamentation of conidia were verruculose (9).

 
 
 
 
 
 
 

References

1. Bensch K, Braun U, Groenewald JZ, Crous PW (2012) The genus Cladosporium. Stud Mycol 72: 1-401.
2. Potin O, Veignie E, Rafin C (2004) Biodegradation of polycyclic aromatic hydrocarbons (PAHs) by Cladosporium sphaerospermum isolated from an aged PAH contaminated soil. FEMS Microbiol Ecol 51: 71-78.
3. Zhdanova NN, Zakharchenko VA, Vember V V, Nakonechnaya LT (2000) Fungi from Chernobyl : mycobiota of the inner regions of the containment structures of the damaged nuclear reactor. Mycol Res 104: 1421-1426.
4. Qiu-Xia C, Chang-Xing L, Wen-Ming H, Jiang-Qiang S, Wen L, et al. (2008) Subcutaneous phaeohyphomycosis caused by Cladosporium sphaerospermum. Mycoses 51: 79-80.
5. Yano S, Koyabashi K, Kato K (2003) Intrabronchial lesion due to Cladosporium sphaerospermum in a healthy, non-asthmatic woman. Mycoses 46: 348-350.
6. De Hoog GS, Guarro J, Gene J, Figueras MJ (2000) Atlas of clinical fungi. 2nd edi. The Netherlands: Centraalbureau voor Schimmekulture.
7. Dixit A, Kwilinski K (2000) 969 Cladosporium sphaerospermum - A new allergic species. J Allergy Clin Immunol 105: S328.
8. Schubert K, Groenewald JZ, Braun U, Dijksterhuis J, Starink M, et al. (2007) Biodiversity in the Cladosporium herbarum complex (Davidiellaceae, Capnodiales), with standardisation of methods for Cladosporium taxonomy and diagnostics. Stud Mycol 58: 105-156.
9. Dugan FM, Schubert K, Braun U (2004) Check-list of Cladosporium names. Schlechtendalia 11: 1-103.
 
 
 
 

 

Cladosporium sphaerospermum UM 843

 
  • Description
  • Morphological
  • References
  • Taxonomic Classification
  • Assembly
  • Gene Models
  • Gene Annotations
  • External Links
  • Sequencing Stats
  • Download Data (Contigs)
  • Download Data (Amino Acids)
  • Download Data (Nucleotides)
  • GBrowse Link
  •  

    Taxonomic Classification

     
    Kingdom: Fungi
    Phylum: Ascomycota
    Class: Dothideomycetes
    Order: Capnodiales
    Family: Cladosporiaceae
    Genus: Cladosporium
    Species: Cladosporium sphaerospermum

     

    Strain Details

     
    Stock Number843
    Morphological IdentityCladosporium sp.
    Molecular IdentityCladosporium sphaerospermum
    Isolation LocationBlood
    Collection Date29/11/2008
    Isolation CountryMalaysia
    AccessionJX966576
     

     

    Assembly

     
    Assembly size(bp): 26,892,198
    Number of contigs: 877
    Number of contigs (>=200bp): 867
    Total contigs size(bp): 26,722,253
    Contigs N50(bp): 92,815
    Contigs GC content(%): 55.67
    Number of scaffolds (>=1000bp): 155
    Total scaffolds size(bp): 26,892,198
    Scaffolds N50(bp): 969,659
    Scaffolds GC content(%): 55.32

    Gene Models

     
    Number of predicted genes (>=99bp): 9,652
    Average gene length: 1,482
    Average number of exons per gene: 2.26
    rRNAs: 42
    tRNAs: 196

    Gene Annotations

     
    KEGG : 999
    EC : 1816
    GO : 6069
    KOG : 5852
    PFAM : 7091
    Interpro : 6841
    CAZyme : 605
    CAZyme Unique : 566
    Secondary Metabolite : 15
    Peptidase : 136
    Peptidase Unique : 134
    Virulence Factor : 405

       
     
  • NCBI Taxonomy
  •  
  • Catalogue of Life
  • Organism Platform Read type Library size(bp) Number of reads Read length(bp) Total read size(bp) Estimated genome size(Mbp) Sequencing coverage(X)
    Cladosporium Illumina HiSeq 2000 Small insert 500 19,253,334 90 1,732,800,060 26.4 - 31.9 55
    Cladosporium Illumina HiSeq 2000 Large insert 5,000 9,987,556 90 898,880,040 26.4 - 31.9 28